Development of sex-associated RAPD markers in wild Pistacia species


Kafkas S. , CETINER S. , PERL-TREVES R.

JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY, cilt.76, ss.242-246, 2001 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 76 Konu: 2
  • Basım Tarihi: 2001
  • Dergi Adı: JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY
  • Sayfa Sayıları: ss.242-246

Özet

Marker assisted selection may greatly facilitate pistachio rootstock breeding as well as cultivar improvement, because of the long juvenile period of Pistacia species. Early diagnosis of seedling sex type would assist breeding and nursery-management in these dioecious species. We searched for RAPD markers linked to sex in P. atlantica, P. terebinthus and P. eurycarpa, the main wild species in Turkey that are used as rootstocks for P. vera. For this purpose, leaf samples were collected from male and female individual trees from each species and sex-pooled DNA samples were prepared by mixing the DNA of ten male and ten female individuals, to screen for sex associated RAPD bands. A total of 472 primers have been screened so far and two bands, amplified by primers BC156 and BC360, appeared to be sex assocaited in P. eurycarpa. The bands were tested in 30 male and 37 female individuals. Band BC156((1300)) was present in all, except one, female trees and was absent from all the male trees. Band BC360((500)) was amplified in 31 out of 37 females and was absent from all the males. In P. atlantica, one primer, OPAK09, amplified a female-associated band (850 bp), that was present in ah 46 female individuals tested and absent in all the 38 male trees tested. it is likely that these markers are linked to sex-determining loci. The sex determination mechanism has not been characterized in Pistacia and segregating populations from controlled crosses are required to elucidate such mechanism and also to measure the genetic distance of our markers from the putative sex loci.