Although the plant root system is one of the most important plant parameters affecting nutrient uptake by plants, root studies in field experiments are rarely conducted in plant nutrition and fertility studies. Since collection of root samples and measurements are difficult and time consuming, they are not considered as a routine plant parameter. Therefore, the effect and importance of the corn root system on plant nitrogen (N) uptake and grain yield was studied under field conditions in Adana, Turkey. Nitrogen was applied at rates of 200, 250, 300, and 350 kg N ha(-1) as urea in a randomized complete block design experiment-with three replications. During course of the experiment, soil, plant, root, and grain samples were collected and prepared for chemical analysis. Nitrogen uptake by plants was predicted using a COMP8 mathematical computer model and compared to actual plant uptake. Grain yield and leaf N content increased with increasing N rates, but root length did not change statistically, Predicted N uptake increased with added N, but was much smaller than observed N uptake under field conditions. Consequently, additional soil and plant parameters should be considered in nutrient uptake models to make the prediction more sensitive.