Laparoscopic artificial insemination in dairy sheep with chilled semen stored for up to 26 h

Gimenez-Diaz C., EMSEN E., Ocak S., Aslan F.

AFRICAN JOURNAL OF BIOTECHNOLOGY, vol.10, no.30, pp.5812-5814, 2011 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 10 Issue: 30
  • Publication Date: 2011
  • Journal Name: AFRICAN JOURNAL OF BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Aquatic Science & Fisheries Abstracts (ASFA), Biotechnology Research Abstracts, CAB Abstracts, Pollution Abstracts, Veterinary Science Database
  • Page Numbers: pp.5812-5814
  • Çukurova University Affiliated: Yes

Abstract

Adult East Freisan crossbred (n = 220) and Chios ewes (n = 105) were divided into four groups and inseminated with chilled semen, which had been stored for 7, 13, 20 or 26 h at 5 degrees C. Unilateral intrauterine insemination (50 x 10(6) spermatozoa in 0.25 ml) was performed with the aid of a laparoscope. Inseminations were carried out 48 - 52 h after pessary removal (30 mg FGA) without detecting estrus. The lambing rates after intrauterine insemination with chilled semen were found to be similar in East Fresian crossbred (40%) and Chios ewes (30%). Intrauterine insemination with chilled semen stored up to 26 h resulted in similar lambing rates; whereas, fertility of Chios ewes tended to decline with increased holding time of chilled semen. From this study, it is concluded that decreasing the storage time of chilled semen at 5 degrees C improves pregnancy in Chios ewes and that East Fresian crossbred ewe's conception rates to intrauterine insemination with chilled semen was relatively higher than Chios ewes.