Effect of sodium butyrate on some alternative splicing genes and BACE1 isoforms in Sh-Sy5y cell line

Ay G., Lüleyap H. Ü., Evyapan G., Pazarcı P., Alptekin D., Pazarbaşı A., ...More

CUKUROVA MEDICAL JOURNAL, vol.46, no.2, pp.764-771, 2021 (ESCI)

  • Publication Type: Article / Article
  • Volume: 46 Issue: 2
  • Publication Date: 2021
  • Doi Number: 10.17826/cumj.870361
  • Journal Name: CUKUROVA MEDICAL JOURNAL
  • Journal Indexes: Emerging Sources Citation Index (ESCI)
  • Page Numbers: pp.764-771
  • Çukurova University Affiliated: Yes

Abstract

Purpose: The aim of this study is to investigate effect of sodium butyrate (NaB), which is a Histon Deacetylase (HDAC) inhibitor, on BACE1 isoforms BACE1/501, BACE1/457 and BACE1/432 and alternative splicing factors including hnRNP H, U2AF35, U2AF65, SRSF1, SRSF2, SRSF5 and SRSF6. Materials and Methods: In this study, SH-SY5Y cells were treated with of 1 mM and 5 mM NaB. Then Real-Time PCR method was performed to evaluate the change in expression of alternative splicing genes and BACE1 isoforms. Results: At 5 mM NaB concentration, a relative decrease of 35% to 80% in expression of the SRSF6, SRSF1, SRSF2, SRSF5 and U2AF65 genes were detected. Morever 5 mM NaB concentration increased expression of BACE1/501, BACE1457 and BACE1/432 isoforms. As NaB concentration increase, expression of SRSF6 mRNA decreased to a lower extent than others. Conclusion: According to our results, it can be concluded that contribution of the U2AF65 and SRSF6 genes to the increase of BACE1/457 and BACE1/432 expression is higher than the other genes.