Investigation of the polymorphism of the myostatin gene by using PCR-RFLP in two tilapia species (Oreochromis aureus and Oreochromis niloticus)

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Baylan M., Mazı G., Özcan N., Özcan B. D., Güzel A. İ.

Journal of Food, Agriculture and Environment, vol.13, no.2, pp.228-231, 2015 (Peer-Reviewed Journal)

  • Publication Type: Article / Article
  • Volume: 13 Issue: 2
  • Publication Date: 2015
  • Journal Name: Journal of Food, Agriculture and Environment
  • Journal Indexes: CAB Abstracts, Food Science & Technology Abstracts, Veterinary Science Database
  • Page Numbers: pp.228-231
  • Çukurova University Affiliated: Yes

Abstract

The aim of the study was to investigate the restriction fragment length polymorphism (RFLP) of the exon 1–3 fragments from the myostatin gene in two tilapia species, Oreochromis aureus, and Oreochromis niloticus. Tissue samples were collected from a total of 50 fish, and the total genomic DNA was extracted and used for PCR amplification. Particular regions of exon 1–3 from the myostatin gene (390, 435, and 342 bp, respectively) were amplified with specific primers for each exon region by using PCR. The PCR-amplified products of each exon region were digested with appropriate restriction endonuclease enzymes and analyzed using agarose gel electrophoresis.