Investigation of the polymorphism of the myostatin gene by using PCR-RFLP in two tilapia species (Oreochromis aureus and Oreochromis niloticus)
Journal of Food, Agriculture and Environment, cilt.13, sa.2, ss.228-231, 2015 (Hakemli Dergi)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 13 Sayı: 2
- Basım Tarihi: 2015
- Dergi Adı: Journal of Food, Agriculture and Environment
- Derginin Tarandığı İndeksler: CAB Abstracts, Food Science & Technology Abstracts, Veterinary Science Database
- Sayfa Sayıları: ss.228-231
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Çukurova Üniversitesi Adresli: Evet
Özet
The aim of the study was to investigate the restriction fragment length polymorphism (RFLP) of the exon 1–3 fragments from the myostatin gene in
two tilapia species, Oreochromis aureus, and Oreochromis niloticus. Tissue samples were collected from a total of 50 fish, and the total genomic
DNA was extracted and used for PCR amplification. Particular regions of exon 1–3 from the myostatin gene (390, 435, and 342 bp, respectively) were
amplified with specific primers for each exon region by using PCR. The PCR-amplified products of each exon region were digested with appropriate
restriction endonuclease enzymes and analyzed using agarose gel electrophoresis.