7th International Mediterranean Scıence and Engıneerıng Congress October 24-26, 2022 Alanya Lonıcera Resort & Spa Hotel, Alanya / Turkey pp152-153., Antalya, Türkiye, 24 - 26 Ekim 2022, cilt.1, ss.151-152
Known as the Neem tree on Earth, Azadirachta Indica is an evergreen plant species. The homeland of the
tree, which has four different species, is India, Burma, Pakistan, South Asia and Australia. Melia Azaderach, which is another
species in our country, grows naturally in tropical climate regions, the main used parts of the rosary tree are the fruits and
leaves, which turn light yellow when ripe. The fruits reach morphological maturity in September-October. Neem (rosary
tree) oil is available in the market as products obtained from these fruits, and its powder is obtained from both fruits and
leaves and used in organic agriculture, medicine and cosmetic products. Depending on the extraction method, neem oil
contains 300 ppm to 2500 ppm of azaderachtin active substance. Azaderachtin, which is a tetratriterponoid used against
insects, is used as a biopesticide in organic agriculture due to its repellent, feeding inhibitory, fertility reducing, sterilizing,
egg laying, development and growth inhibitory effects in insects. Natural insecticides of plant origin constitute 1% of the
world insecticide market, and annual sales increase by 10-15% every year due to the increase in organic farming. Neem oil
is imported to our country from India and other countries. On average, 1 litre of neem oil is sold for 900 TL. In this study,
it is planned to bring the variants of the Indica species, which are used in the production of neem oil and are not found in
our country, to be reproduced by tissue culture and to use the neem oil extracted from the seeds of these variants in the
production of an Azadirachtin A isomer-based pesticide. The study is carried out within the scope of the project numbered
TAGEM-21/AR-GE/11.
Keywords: Azadirachta Indica, Neem, Oil, Bio-Pest