INTRA- AND INTERSPECIFIC RECOMBINATION IN THE NATURAL CONDITION OF PEPPER VEIN YELLOWS VIRUS AND RESPONSE OF PVR ALLELES IN PEPPER


Arpacı B. B.

Applied Ecology And Environmental Research, vol.17, no.6, pp.14653-14664, 2019 (SCI-Expanded)

Abstract

Since their first observing in Japan, poleroviruses have threatened pepper cultivation all around the World. Symptoms of vein yellowing originating from poleroviruses have become widespread in both open field and protected pepper cultivation areas recently. In this work samples collected from highly infected pepper fields in South East Turkey have been determined as Pepper Vein Yellows Virus (PVYV) and Beet Western Yellows Virus (BWYV) (genus Polerovirus, family Luteoviridae). Recombinant PVYV isolates have been observed from intra and interspecific combination in relation to part of the RNA-dependent RNA polymerase coding region subjected to recombination detection methods. One of the PVYV isolates has induced recombination between BWYV and PVYV and located middle of the PVYV and BWYV isolates. Another isolate recombined from intraspecific combination has been transferred onto pepper genotypes carrying pvr alleles and landraces under both artificial and natural conditions to determine their response. None of the pvr alleles in pepper genotypes used in this study could contribute to resistance to PVYV isolates.