Detection a novel genetic variant of the Myf5 gene in Turkish Anatolian Water Buffalo

BAYRAKTAR M.

PROGRESS IN NUTRITION, cilt.24, sa.2, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 24 Sayı: 2
  • Basım Tarihi: 2022
  • Doi Numarası: 10.23751/pn.v24i2.12184
  • Dergi Adı: PROGRESS IN NUTRITION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, EMBASE
  • Anahtar Kelimeler: Anatolian Buffalo, Myf5, sequencing, SNPs, BsuRI, GROWTH TRAITS, CANDIDATE GENES, MEAT QUALITY, ASSOCIATION, POLYMORPHISM, YIELD, CARCASS, MARKERS
  • Çukurova Üniversitesi Adresli: Evet

Özet

Myf5 gene belongs to myogenic regulatory factors (MRFs) families and has an active role in muscle differentiation and the development of skeletal muscle cells. The study aimed to detect genetic variants of the Myf5 gene in Anatolian buffaloes. PCR-RFLP and DNA sequencing methods were used to detect SNP regions of the Myf5 gene. Seven restriction enzymes were used to identify the polymorphic regions. Six enzymes (RsaI, SspI, DraI, HinfI, PstI, and EcoRI) showed monomorphic regions and only, BsuRI enzyme showed a polymorphic region in the intron 2 site. It was detected a novel SNP in the C14553A site in the second intron of the Myf5 gene in Anatolian buffaloes. These novel findings may be used as a genetic marker and contribute to the performance of buffaloes in the future.