The effects of chitosan extracted from shrimp (Metapenaeus stebbingi) shell waste and commercial chitosan on fatty acid profile of European eel (Anguilla anguilla) fillet pieces during refrigerated storage were investigated. Fish pieces samples were treated with aqueous solution of 0.1 and 1% extracted and commercial chitosan, and then stored at 4 degrees C for 18 days. The fillet pieces samples were analyzed periodically for fatty acid analysis. No statistically significant change was detected in the total saturated fatty acid (SFA) contents of eel fillet pieces in the all groups at the end of storage (p>0.05). There were no significant difference in monounsaturated fatty acid (MUFA) and polyunsaturated fatty acid (PUFA) contents of the groups added 1% extracted and commercial chitosan (p>0.05), while a percentage decrease was determined in MUFA and PUFA contents of groups control, added 0.1% extracted and commercial chitosan at the end of storage (p<0.05). It was concluded appropriate to use chitosan obtained from shrimp shells not evaluated and discarded as waste products in Turkey during the storage of fatty fish like eel.