Research Information System
Monatshefte fur Chemie, 2025 (SCI-Expanded)
Determination of iron in serum is of great importance in the diagnosis, screening, and follow-up of various iron metabolism diseases, especially anemia. In this matter, biosensors meet the systems needed to detect iron levels. Regarding the importance of determining the iron level in each human plasma, however, the role of voltammetric biosensors has not been paid attention to by the researchers. To fulfill this shortcoming, this paper aims to develop a voltammetric biosensor using hydrogen peroxidase (HRP) enzyme immobilized to the bioactive layer for the measurement of serum iron amount. To accomplish this aim, the bioactive layer was first prepared by immobilizing HRP, bovine serum albumin (BSA), gelatin, and glutaraldehyde on the gold electrode with UV light. To ensure the separation of Fe3+ in the serum from the transferrin, acetate buffer having a pH of 5.0 was preferred. Then, hydroxylamine hydrochloride was employed to reduce the Fe3+ to Fe2+. Fe2+ produced as a result of the reduction which was measured by the reaction of hydrogen peroxidase enzyme. This serum iron biosensor indirectly can measure the Fe2+ concentration. Iron determination was performed in 25 serum samples with the developed electrode, and a positive correlation of 0.865 and P < 0.05 was observed in the comparison study. According to the obtained results, the developed biosensor was a reliable method in serum iron analysis. Moreover, it is biocompatible for studies in biological fluids, which can be converted into a portable digital device such as glucose meters that measure blood sugar.