In-vitro multiplication of clonal apple rootstocks M-9, M-26 and MM-106 by meristem culture

Aklan K., Cetiner S., Aka-Kacar Y. , Yalcin-Mendi Y.

5th International Symposium on Temperate Zone Fruits in the Tropics and Subtropics, Adana, Turkey, 29 May - 01 June 1996, pp.325-327 identifier identifier


Meristems of apple rootstocks M-9, M-26 and MM-106 were cultured on 1/2 strength MS medium containing 2.2 mu M BA, 0.5 mu M IBA, and 0.3 mu M GA3. M-26 and MM-106 shoots were transferred on Lepoivre and MS media, respectively, supplemented with 4.4 I-IM BA, 5 mu M IBA, 0.3 IJ-M GA3. The rate of shoot proliferation per mother explant of M-26 and MM-106 were 6 and 3.4, respectively. The best rooting of MM-106 was achieved with the use of 4 mu M IRA, 0.3 mu M giberellic acid (GA3) and 1300 mu M phloroglucinol (PG) in which 80 % rooting was obtained. During the proliferation phase, shoot tip necrosis and vitrified shoots were observed with MM-106. To overcome these, PG was used. Although vitrification was not observed, the shoot elongation M-26 was limited to around 1 cm. In M-9, insufficient shoot proliferation was obtained, thus experiments are underway to increase shoot production.