A putative role for S-nitrosoglutathione as the source of nitric oxide in photorelaxation of the mouse gastric fundus


Ogulener N. , Ergun Y.

EUROPEAN JOURNAL OF PHARMACOLOGY, cilt.450, ss.267-275, 2002 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 450 Konu: 3
  • Basım Tarihi: 2002
  • Doi Numarası: 10.1016/s0014-2999(02)02098-8
  • Dergi Adı: EUROPEAN JOURNAL OF PHARMACOLOGY
  • Sayfa Sayısı: ss.267-275

Özet

Mouse gastric fundus strips were relaxed by ultraviolet light (UV) irradiation, exogenous nitric oxide (NO), isoproterenol, S-nitrosoglutathione, S-nitroso-L-cysteine and S-nitroso-N-acetyl-penicillamine. Glutathione did not affect relaxations in response to UV irradiation, exogenous NO and isoproterenol while inhibiting that with S-nitrosoglutathione. L-Cysteine inhibited responses to UV irradiation and exogenous NO, but not in the presence of exogenous Cu2+/Zn2+ superoxide dismutase. However, L-Cysteine alone or in combination with Cu2+/Zn2+, superoxide dismutase did not affect the relaxations in response to S-nitroso-L-cysteine. Ethacrynic acid and diamide inhibited photorelaxations but not the relaxations with exogenous NO and isoproterenol. This inhibition was prevented by glutathione, but not by L-cysteine. S-nitrosoglutathione-induced relaxations were abolished by diamide and ethacrynic acid, whereas responses to S-nitroso-L-cysteine and S-nitroso-N-acetyl-penicillamine were only inhibited by ethacrynic acid. These results suggest that S-nitrosoglutathione may, at least in part, be the putative S-nitrosothiol, which is converted to NO in response to UV irradiation in mouse gastric fundus strips. (C) 2002 Elsevier Science B.V. All rights reserved.