In this study we investigated the effect of the nozzle type as one of the most important parts of the open-end rotor spinning system on yarn quality. 100% Urfa Region cotton was used for the production of yarns of 20 tex. Experimental studies were made on ten spinning units of a Rieter R1 machine. Five different ceramic nozzle types were used: K4KK (plain with 4 grooves), K4KS (plain with 4 grooves and aggressive flute insert), K6KF Vain with 6 grooves), K8KK Vain with 8 groves), and KSNX (spiral with soft flute insert). Apart from the nozzle types, the yarn production conditions and machine parameters were kept the same. All production activities were realized under the same mill conditions. For yarn hairiness, yarn evenness and yarn imperfections, an Uster Tester 4SX and for yarn tenacity an Uster Tensorapid 3 tester were used. The test results were analysed using the one way-ANOVA statistical method of Design Expert 6.0.1. In one of the evaluations, it is clearly seen that the nozzle type mostly affects yarn quality and yarn tenacity.