Ethnopharmacological and Antiaging Evaluation of Salvia cadmica: Modulation of MMP1/MMP3 and Collagen in Oxidative Stress-Induced Senescence
Chemistry and Biodiversity, cilt.23, sa.8, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 23 Sayı: 8
- Basım Tarihi: 2026
- Doi Numarası: 10.1002/cbdv.71559
- Dergi Adı: Chemistry and Biodiversity
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Chemical Abstracts Core, EMBASE, MEDLINE, Natural Science Collection (ProQuest), Biomedical Reference Collection: Corporate Edition (EBSCO), Health Research Premium Collection (ProQuest), Materials Science & Engineering Collection (ProQuest), Technology Collection (ProQuest)
- Anahtar Kelimeler: ADMET, gene expression, MM/GBSA, molecular docking, Salvia cadmica, senescence
- Çukurova Üniversitesi Adresli: Evet
Özet
Salvia cadmica, an Anatolian endemic species traditionally used for wound healing and inflammation, has been scarcely investigated for its anti-aging potential. In this study, the aqueous–ethanol extract of S. cadmica was evaluated for its antioxidant, cytoprotective, and anti-senescence effects using in vitro and in silico approaches. LC–ESI–MS/MS analysis identified rosmarinic acid (3447 µg/g), luteolin 7-glucoside (767 µg/g), and luteolin (586 µg/g) as major constituents. The extract exhibited strong antioxidant capacity, particularly in the phosphomolybdenum assay (713.51 mg TEs/g), and showed notable radical scavenging activity in ABTS (140.00 mg TEs/g) and DPPH (99.20 mg TEs/g) assays. In H2O2-induced senescent human dermal fibroblasts (HDFa), pre-treatment with the extract significantly reduced senescence-associated β-galactosidase positivity from 97.15% to 49.12% and improved cell viability. The extract downregulated MMP1 and MMP3 mRNA expression (0.51- and 0.66-fold, respectively) while markedly upregulating COL1A1 expression (7.48-fold). Molecular docking and MM/GBSA analyses revealed that luteolin 7-glucoside showed high binding affinities toward MMP1 and MMP3 (ΔGdocking = −9.06 and −9.86 kcal/mol; ΔGMM/GBSA = −37.21 and −50.22 kcal/mol, respectively). In silico ADMET predictions supported a favorable dermal safety profile. Overall, S. cadmica represents a promising natural source of bioactive compounds with antioxidant and anti-aging potential relevant to skin aging prevention.