The aim of this study was to test nectin-4 by immunohistochemistry as a potential biomarker in enzootic nasal adenocarcinoma (ENA) of goats. Twenty-four archival ENA case samples [from 14 male and 10 female hair goats (Capra hircus)] were used. The samples were stained with haematoxylin and eosin (HE). Nectin-4 expression was studied by immunohistochemistry. By microscopy, tubular, papillary, and mixed patterns of ENA were diagnosed in the cases. Immunohistochemically, the tumours showed moderate nectin-4 expression (++) in 14 cases (58.3%), strong expression (+++) in five cases (20.8%), and weak expression (+) in three cases (12.5%), while two cases (8.3%) were negative. Normal nasal tissues were not stained with nectin-4. The results suggest that nectin-4 may be used as a valuable biomarker of ENA.