Molecular Characterization of Almond Cultivars Using Simple Sequence Repeat Markers


Esgandaripirmorad F., KARCI H. , PAİZİLA A. , Topcu H., KAFKAS S.

ERWERBS-OBSTBAU, 2022 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Publication Date: 2022
  • Doi Number: 10.1007/s10341-022-00640-7
  • Title of Journal : ERWERBS-OBSTBAU
  • Keywords: Almond, SSR, Prunus dulcis, Genetic dissimilarity, Relationship, PERSICA L. BATSCH, GENETIC DIVERSITY, MICROSATELLITE MARKERS, LINKAGE MAP, SSR MARKERS, PEACH, GERMPLASM, GENOTYPES, POLYMORPHISM, RELATEDNESS

Abstract

Genetic diversity analysis of twenty-two almond cultivars originated from eight different countries was performed to determine genetic relationship and variation between them using simple sequence repeat (SSR) markers. A total of 28 SSR loci were chosen from 40 SSRs based on their polymorphism levels. A total of 265 alleles were generated from 28 SSR loci ranging from 2 to 14 alleles with an average of 9.46 per locus. According to genetic diversity analysis, the mean value of the effective number of alleles, observed heterozygosity and expected heterozygosity were calculated as 5.85, 0.75 and 0.76, respectively. The highest number of alleles was obtained from the CPDCT042 locus with 14 alleles. The highest expected heterozygosity (0.90) polymorphism information content values were obtained from the CPDCT042 locus. Additionally, 21 SSR loci produced specific alleles for 17 cultivars. The almond cultivars were separated into three main clusters based on UPGMA (unweighted pair group method with arithmetic averages), PCoA (principal component analysis) and STRUCTURE analysis. The cultivars originated from Turkey grouped in one cluster, while the other cultivars were in the remaining two clusters. The highest genetic distance was calculated between 'Nurlu' and 'Cristomorto' cultivars, while 'Nonpareil' and 'Sonora' were the closest ones. Consequently, the results in this study will provide useful information for fingerprinting of cultivars, future breeding and genetic studies in almond.