Optimization of culture conditions for alkaline protease production from waste breads using Bacillus subtilis


Ozdenefe M. S., DİNÇER S., ÜNAL M. Ü., Kayis F. B., Takci H. A. M., Arkut A.

ROMANIAN BIOTECHNOLOGICAL LETTERS, cilt.22, sa.3, ss.12597-12610, 2017 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 22 Sayı: 3
  • Basım Tarihi: 2017
  • Dergi Adı: ROMANIAN BIOTECHNOLOGICAL LETTERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED)
  • Sayfa Sayıları: ss.12597-12610
  • Çukurova Üniversitesi Adresli: Evet

Özet

An alkaline protease-producing microorganism was isolated from a soil sample which is collected from Cukurova University campus and identified as a strain of Bacillus subtilis. The culture conditions were optimized for maximum enzyme production. Optimum enzyme production was achieved from fermentation medium which includes 1% waste bread and 1% casein powder as carbon and nitrogen source at pH 10.5 and 37 degrees C for 72 h incubation period with agitation of 200 rpm. The optimum inoculum amount and inoculum age were found as 5% and 4 hrs, respectively. Enzyme production reached its maximum value when a baffled Erlenmeyer flask with cotton was used. The approximately 20-fold increase in alkaline protease production was observed as a result of culture medium optimization. Substantial increase in alkaline protease activity was detected in optimized medium (134.64 U/mL) when compared with unoptimized medium (6.45 U/mL). It has been concluded that screening of suitable medium components has asignificant role in the production of alkaline protease by B. subtilis.