Akademik Veri Yönetim Sistemi
Tez Türü: Doktora
Tezin Yürütüldüğü Kurum: Erciyes Üniversitesi, Veteriner Fakültesi, Klinik Bilimler, Türkiye
Tez Danışmanı: Tayfur Bekyürek
Tezin Onay Tarihi: 2018
Tezin Dili: Türkçe
Desteklendiği Program: Diğer
Özet:
In this study, gonadotropin and exogenous progesterone
administration following superovulation application and superovulation
applications with traditional methods the effects on the quality and number of
embryos obtained were investigated in cattle.
In the study, 20 Holstein cows who aged 3 years were
divided into two groups. In Group I to animals were injected with cloprostenol
for interval with 11 days and from day 9 of the cycle, injections of FSH were
performed at decreasing doses for four days twice daily. 5th and 6th
FSH injections with cloprostenol was administered.
Animals in Group II were administered HCG, after 7
days cloprostenol and buserelin injection on the 7th day of the
following cycle. Intravaginal device containing
progesterone was placed on the 9th day of the cycle, FSH and
cloprostenol were administered as in Group I from the fourth day after the
administration of exogenous progesterone in Group II. In Group II, the intravaginal
progesterone-containing device was removed after the 6th FSH
injection.
In both groups, were seeded three times with an
interval of 12 hours after the last FSH injection. Buserelin injection was
performed with the second artificial insemination. Embryos
were collected on the 7th day after insemination and quality and
developmental stages were evaluated.
At
the end of the research, the average number of the total follicle, corpus luteum, ova/embryos, embryos, transferable embryos,
1st and 2 nd qrade embryos were evaluated and numbers were found as 2.00±0.88,
8.50±1.59, 7.50±1.99, 6.00±2.00, 5.50±1.84, 4.40±1.46 ve 1.0±0.47; in Group I; 2.60±0.64, 9.40±1.00, 7.00±1.61, 6.10±1.47, 5.50±.1.59, 4.90±1.37 ve
0.6±0.30 in Group II, respectively (P>0,05).
In conclusion, no statistically significant between the two protocols was found in terms of embryo quality and number. However the difference between the groups in terms of division rates is statistically significant. The rate of cleavage in Group II was higher than in Group I.
Keywords: Superovulation, Folliculer Wave, GnRH, Embryo, Cattle, P4