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Turk Hijyen ve Deneysel Biyoloji Dergisi, vol.82, no.1, pp.3-12, 2025 (Scopus)
Objective: Kisspeptin (KP) and neurokinin (NK) are neuropeptides that govern the reproductive endocrine axis through regulating hypothalamic neuronal activity and pulsatile. Their critical role in reproductive health was first described by the demonstration of congenital hypogonadotropic hypogonadism and pubertal developmental anomalies following loss of function due to mutations in genes encoding the KP or NK signaling. This study aimed to investigate the efficacy of KP and NK, in mesenchymal stem cell (MSC)-based treatment of different origin, and examine the relationship of the expression of these genes with the passage number. Methods: Three adult rats (Rattus norvegicus) were used in our study. MSCs were obtained from bone marrow and adipose tissue of rats. The MSCs were analysed by flow cytometry using surface markers and RNA (ribonucleic acid) isolation was performed. Gene expressions of KP, NK and their receptors were investigated by real-time polymerase chain reaction (RT-PCR) and their relationship with the passages number was compared. Welch paired t-test was used to compare the expression of each gene at different passages. Statistical significance was accepted as 0.05 (p≤0.05). Results: The cultured bone marrow and adipose tissue cells were determined to be MSCs. When real-time PCR results of bone marrow-derived MSCs were evaluated, it was observed that kisspeptin receptor gene expression increased 14-fold in the sixth passage and 29-fold in the seventh passage compared to the fourth passage (p≤0.05). In the tachykinin gene, it was found that gene expression was almost completely suppressed in the fifth passage compared to the fourth passage (p≤0.05). In the tachykinin receptor gene, it was determined that gene expression was completely induced in the sixth passage, compared to the fifth passage (p≤0.05). When adipose tissue-derived MSC RT- PCR results were evaluated, it was observed that tachykinin receptor gene expression increased 15-fold, tachykinin gene expression increased 30-fold in the fifth passage, and KP receptor gene expression increased three-fold in the seventh passage compared to the fourth passage (p≤0.05). Conclusion: MSCs can be obtained from an easily available tissue such as bone marrow and adipose tissue is thought to shed light on the medical science for the future. In addition, it was concluded that the number of passages should be considered when analyzing the expressions of KP and NK genes, which have a very important role in the transition to puberty, and the number of passages should be selected by considering the gene expressions in the passage to be used.