Synthetic cannabinoids (SCs) are the most rapidly growing class of recreational designer drugs. Illicit drug manufacturers began to produce herbal smoking materials under a variety of brands names, e.g. ?Spice, K2, Bonsai, Yucatan Fire?. They were appeared on the European market in 2008. In this study, types of SCs in the herbal product sold as ?Bonsai? in Turkey were determined and the identification of these substances in biological samples collected from rats depending on the inhalation of different amounts of plant material were aimed.