Simple Extraction and Rapid HPLC Method for Tocopherol Analysis in Marine and Fresh-water Fish Species

ÖZOĞUL F. , ÖZOĞUL Y. , Kuley E.

FOOD SCIENCE AND TECHNOLOGY RESEARCH, vol.17, no.6, pp.595-598, 2011 (Journal Indexed in SCI) identifier

  • Publication Type: Article / Article
  • Volume: 17 Issue: 6
  • Publication Date: 2011
  • Page Numbers: pp.595-598
  • Keywords: fresh water fish, marine fish, tocopherol, HPLC method, extraction procedure, PERFORMANCE LIQUID-CHROMATOGRAPHY, ALPHA-TOCOPHEROL, GAMMA-TOCOPHEROL, VITAMIN-E, OXIDATION, PRODUCTS, OIL


A rapid high-performance liquid chromatographic (HPLC) method with simple extraction procedure for the determination of tocopherols in seafood was developed. A continuous isocratic elution system was used for the analysis with a mixture of acetonitrile and methanol. Tocopherols were identified by comparison of retention times and peak area values with standard of alpha-, beta-, gamma- and delta-tocopherols. The HPLC technique used showed good performance parameters (linearity r(2) = 0.9999, and relative standard deviation (RSD) <= 12.38%). The total recoveries for each tocopherol were 99.7, 99.9, 99.8 and 99.7% for alpha, beta, gamma- and delta, respectively. This method was then applied to freshwater and seawater fish species to detect their tocopherol contents. Significant differences (P < 0.05) were observed in the content of tocopherol among fish species. The highest content was obtained from shrimp (81.3 mg/kg), while the lowest was found for whiting (9.8 mg/kg).