Effect of Acute Gabapentin on Open Field Test Behavior in Balb/c mice Administered with MK-801

Dönmez Kutlu M., Akıllıoğlu K.

ACTA PHYSIOLOGICA, vol.227, no.S722, pp.93, 2019 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 227 Issue: S722
  • Publication Date: 2019
  • Doi Number: 10.1111/apha.13424
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.93
  • Çukurova University Affiliated: Yes


AIM: In our study, the effect of acute gabapentin on locomotor

activity and anxiety-like behavior in Balb/c mice administered

with MK-801 was evaluated in the open-field test.

METHODS: In present study, 8-10 weeks old 19 male adult

Balb/c mice were used. Mice were administered

uncompetetive N-methyl-D-aspartate (NMDA) receptor

blocker MK-801 (0.5mg/kg dose, 0.1mL/kg body weight). The

same volume of saline (0.9%NaCl) was injected

intraperitoneally to the mice in the control group. Gabapentin

was administered at a dose of 100 mg/kg 30 min. after MK-

801 administration. Mice were tested with open-field test 30

min. after injection. The locomotor activity and anxiety-like

behavior of the mice were recorded with a video-camera for 5

min and scored with the ETHOVISION XT program. Data

were analyzed using Mann-Whitney-u test was used following

Kruskal-Wallis. Ethics committee approval C.U. animal

experiments were taken from the local ethics committee with

the decision number 3/8, dated 09.05.2019.

RESULTS: The time spent in the center of the MK-801 group

compared with the control group increased, latency to the

center and the frequency of rearing decreased (p<0.05).

Gabapentin administration after MK-801 injection decreased

the frequency of rearing in mice compared to the group of

administered gabapentin (p<0.05) and did not significantly

change the latency to the center and the time spent in the


CONCLUSION: MK-801-induced NMDA blockade reduced

anxiety-like behavior in the open-field test. Gabapentin did not

change this effect of MK-801.In conclusion, it can be argued

that the effects of NMDA receptor blockade in the open-field

test cannot be altered by the increase in acute GABA level.

Keywords: Balb/c mouse, gabapentin, MK-801, open field

test, schizophrenia.