Biological and Molecular Detection of Citrus Psorosis Virus in Citrus in the Eastern Mediterrenean Region of Turkey

Kayim M.

JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY, vol.19, no.2, pp.259-262, 2010 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 19 Issue: 2
  • Publication Date: 2010
  • Doi Number: 10.1007/bf03263352
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.259-262
  • Keywords: Biological indexing, CPsV, C. clementina, C. reticulata, C. sinensis, RT-PCR
  • Çukurova University Affiliated: Yes


Fifty bud sticks exhibiting vein flecking, vein clearing and oak-leaf pattern symptoms of 'Satsuma' and 'Clementine' mandarins, and 'Washington navel' and local oranges in the field in Mersin, Kozan and Adana provinces were collected and grafted on one-year-old sour orange seedlings to maintain Citrus psorosis virus (CPsV) in indicators. Thirty two indicator plants showed typical symptoms of vein flecking, vein clearing, and vein banding with oak-leaf pattern. Eighteen plants gave mild leaf symptoms. CPsV was detected by RT-PCR from CPsV-infected sources. Leaf samples for RT-PCR were collected from symptomatic field trees. In all, thirty samples from different trees of mandarin and twenty samples from different trees of sweet oranges were collected. A 434 bp specific DNA for the coat protein was amplified from the cDNA of CPsV-infected leaf samples. This specific DNA product was not amplified from healthy leaf samples or asymptomatic leaves collected from CPsV-infected trees.