Determination of ethyl glucuronide in human hair by hydrophilic interaction liquid chromatography-tandem mass spectrometry


Yaldiz F., DAĞLIOĞLU N. , HİLAL A. , Keten A., GÜLMEN M. K.

JOURNAL OF FORENSIC AND LEGAL MEDICINE, vol.20, no.7, pp.799-802, 2013 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 20 Issue: 7
  • Publication Date: 2013
  • Doi Number: 10.1016/j.jflm.2013.06.013
  • Title of Journal : JOURNAL OF FORENSIC AND LEGAL MEDICINE
  • Page Numbers: pp.799-802

Abstract

Ethyl glucuronide (EtG) is a direct metabolite of ethanol and has been utilized as a marker for alcohol intake. This study presents development, validation and application of a new hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) method for the analysis of EtG in human hair samples. The linearity was assessed in the range of 5-2000 pg/mg hair, with a correlation coefficient of >0.99. The method was selective and sensitive, with a limit of detection (LOD) and limit of quantitation (LOQ) of 0.05 pg/mg and 0.18 pg/mg in hair, respectively. Differently from the extraction procedures in the literature, a fast and simple liquid-liquid method was used and highest recoveries and cleanest extracts were obtained.