Genetic relationship of grape cultivars by ISSR (Inter-Simple Sequence Repeats) markers

SABIR A., KAFKAS S., TANGOLAR S., BÜYÜKALACA S.

EUROPEAN JOURNAL OF HORTICULTURAL SCIENCE, cilt.73, sa.2, ss.84-88, 2008 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 73 Sayı: 2
  • Basım Tarihi: 2008
  • Dergi Adı: EUROPEAN JOURNAL OF HORTICULTURAL SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.84-88
  • Anahtar Kelimeler: grapevine, genetic similarity, ISSR, genetic analysis, VITIS-VINIFERA L, RAPD MARKERS, LINKAGE MAP, IDENTIFICATION, PCR, DISCRIMINATION, POLYMORPHISM, GERMPLASM, AFLP, L.
  • Çukurova Üniversitesi Adresli: Evet

Özet

The ISSR (Inter-Simple Sequence Repeats) data were used to assess genetic relationship among 16 table grape varieties (Vitis ssp.) grown in Turkey. Fourteen primers selected from 50 primers generated a total of 110 clear DNA fragments in which 88 were polymorphic (80.5%). The mean similarity index of all pair-wises was 0.637, and ranged from 0.422 ('Isabella' and 'Konigin der Weingarten') to 0.902 ('Alphonse Lavallee' and 'A. L. Type Royal'). Consequently, considering morphological and cultural characteristics including the high similarity value, 'Alphonse Lavallee' and 'A. L. Type Royal' could be theoretically identical. Grape cultivars analysed were divided on the clustering dendrogram (by UPGMA) according to genetic composition of related species. Our data deduced that distant genetic similarity was found between groups of different cultivars of species. 'Isabella' (V. labrusca) and 'Kyoho' (V. vinifera x V. labrusca, 4x) had a far similarity to V vinifera grapes.