Optimization of lipase-catalyzed synthesis of fructose stearate using response surface methodology

Tukel S., Sahin P. B. , YILDIRIM D.

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, vol.41, no.5, pp.344-351, 2013 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 41 Issue: 5
  • Publication Date: 2013
  • Doi Number: 10.3109/21691401.2012.743899
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.344-351


In this study, immobilized lipase-catalyzed esterification reaction between fructose and stearic acid was examined for the synthesis of a useful compound, fructose stearate, using response surface methodology. The increase of water content in the reaction medium was the negative effect while the increase in initial stearic acid/fructose molar ratio was the greatest positive effect on the yield. The highest fructose stearate yield was obtained as 65% in tert-butanol. The product yield was enhanced in 1-butyl-3-methylimidazolium trifluoromethanesulfonate obtained as 74% under the optimized conditions. The spectroscopic and elemental analysis methods showed that the esterification reaction is regioselective and the product is fructose monostearate.