Isolation and characterization of bacteria with antibacterial properties from Nile tilapia (Oreochromis niloticus)

ETYEMEZ M., BALCAZAR J. L.

RESEARCH IN VETERINARY SCIENCE, cilt.105, ss.62-64, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 105
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1016/j.rvsc.2016.01.019
  • Dergi Adı: RESEARCH IN VETERINARY SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.62-64
  • Anahtar Kelimeler: Antimicrobial activity, Adhesion, Potential probiotics, Fish pathogens, PROBIOTICS, AQUACULTURE
  • Çukurova Üniversitesi Adresli: Evet

Özet

One hundred and twenty bacterial isolates were obtained from the intestinal mucus of Nile tilapia (Oreochromis niloticus) and screened for antagonistic activity and adherence abilities. Based on in vitro antagonism against two pathogens (Streptococcus iniae and Edwardsiella piscicida), five isolates were selected and identified by 16S rRNA gene sequence analysis. All antagonistic isolates were affiliated to the genus Bacillus, which showed inhibitory activity against S. iniae. Only the isolate B191 (closely related to Bacillus mojavensis) inhibited the growth of both pathogens. Moreover, isolate B191 adhered significantly better to fish intestinal mucus than other antagonistic isolates. According to our results, these bacterial isolates, particularly isolate B191, should be further studied to explore their probiotic effects under in vivo conditions. (C) 2016 Elsevier Ltd. All rights reserved.