Seed-Derived Microbial Colonization of Wild Emmer and Domesticated Bread Wheat (<i>Triticum dicoccoides</i> and <i>T. aestivum</i>) Seedlings Shows Pronounced Differences in Overall Diversity and Composition.

Özkurt E., Hassani M. A., Sesiz U., Künzel S., Dagan T., Özkan H., ...Daha Fazla

mBio, cilt.11, sa.6, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 11 Sayı: 6
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1128/mbio.02637-20
  • Dergi Adı: mBio
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Pollution Abstracts, Directory of Open Access Journals
  • Anahtar Kelimeler: seed-associated microbiome, plant domestication, plant breeding, microbiota assembly, agriculture, plant microbiota, seed microbiota, wheat domestication, wheat microbiota, BACTERIAL, COMMUNITIES, COEVOLUTION, ENDOPHYTES, EVOLUTION, INTERPLAY, SELECTION, PLANTS, LIFE
  • Çukurova Üniversitesi Adresli: Evet

Özet

The composition of the plant microbiota may be altered by ecological and evolutionary changes in the host population. Seed-associated microbiota, expected to be largely vertically transferred, have the potential to coadapt with their host over generations. Strong directional selection and changes in the genetic composition of plants during domestication and cultivation may have impacted the assembly and transmission of seed-associated microbiota. Nonetheless, the effect of plant speciation and domestication on the composition of these microbes is poorly understood. Here, we have investigated the composition of bacteria and fungi associated with the wild emmer wheat (Triticum dicoccoides) and domesticated bread wheat (Triticum aestivum). We show that vertically transmitted bacteria, but not fungi, of domesticated bread wheat species T. aestivum are less diverse and more inconsistent among individual plants compared to those of the wild emmer wheat species T. dicoccoides. We propagated wheat seeds under sterile conditions to characterize the colonization of seedlings by seed-associated microbes. Hereby, we show markedly different community compositions and diversities of leaf and root colonizers of the domesticated bread wheat compared to the wild emmer wheat. By propagating the wild emmer wheat and domesticated bread wheat in two different soils, we furthermore reveal a small effect of plant genotype on microbiota assembly. Our results suggest that domestication and prolonged breeding have impacted the vertically transferred bacteria, but only to a lesser extent have affected the soilderived microbiota of bread wheat.