Histopathological changes of some resistant and susceptible wheat cultivars caused by plant parasitic nematodes

Kasapoglu Uludamar E. B. , KARABIYIK Ş. , ELEKCİOĞLU İ. H.

TURKIYE ENTOMOLOJI DERGISI-TURKISH JOURNAL OF ENTOMOLOGY, vol.44, no.1, pp.91-100, 2020 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 44 Issue: 1
  • Publication Date: 2020
  • Doi Number: 10.16970/entoted.621047
  • Page Numbers: pp.91-100


Root lesion nematodes, Pratylenchus thornei Sher & Allen, 1953 and Pratylenchus neglectus (Rensch, 1924) (Tylenchida: Pratylenchidae), and cereal cyst nematode, Heterodera avenae Wollenweber, 1924 (Tylenchida: Heteroderidae) are economically important migratory and sedentary endoparasites on host plant roots. In this study, cellular variations in roots of resistant and susceptible wheat cultivars infected by P. thomei, P. neglectus and H. avenae were investigated between 2017 and 2018. Wheat cultivars were infected with P. neglectus, P. thornei, and H. avenae, separately and H. avenae and P. thomei together. Twelve weeks after inoculation, roots were washed, embedded in paraffin, cut by microtome. The root cell slides were examined under light microscope for histopathological changes in the root cells. The root lesion nematodes moved from epidermal to cortical cells by damaging the cells along the feeding path. Due to the nematode infection, cortical cells of most wheat cultivars were destroyed by the development of many cavities in the root tissue. Also, it was observed that many root lesion nematodes fed collectively on the same site either stretched out or coiled in different cell layers. Heterodera avenae fed on the cortex cells as did root lesion nematode.