The effects of exercise on skeletal and heart muscle citrate synthase and carnitine palmitoyltransferase mRNA expressions in high-calorie fed rats

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Günaşti Ö., Özdemir Ç., Özgünen K. T., Yilmaz M. B., Öksüz H., Zorludemir S., ...Daha Fazla

GENERAL PHYSIOLOGY AND BIOPHYSICS, cilt.40, sa.4, ss.341-350, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 40 Sayı: 4
  • Basım Tarihi: 2021
  • Doi Numarası: 10.4149/gpb_2021019
  • Dergi Adı: GENERAL PHYSIOLOGY AND BIOPHYSICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, EMBASE, MEDLINE
  • Sayfa Sayıları: ss.341-350
  • Anahtar Kelimeler: Aerobic exercise, High-fat diet, Mitochondria, Lipid droplets, FATTY-ACID OXIDATION, GENE-EXPRESSION, INSULIN-RESISTANCE, LIPID-METABOLISM, OBESITY, ENDURANCE, DIET, COA, MITOCHONDRIA, ADAPTATIONS
  • Çukurova Üniversitesi Adresli: Evet

Özet

In this study, the effect of high-calorie feeding and aerobic exercise on skeletal and cardiac muscle citrate synthase (CS), carnitine palmitoyltransferase- I (CPT-I), and -II (CPT-II) mRNA expressions were evaluated. Genetically non-obese rats were grouped as normal-high calorie and sedentary-exercising. Gastrocnemius-soleus and heart muscles' CS, CPT-I, and CPT-II expressions and skeletal muscles' histopathological characteristics were evaluated. High- fat diet had increased body weight by 10% and aerobic exercise did not make any difference. Skeletal muscle CS expression was increased significantly in normal-calorie exercising group. Exercise and high-fat diet did not change CPT-I and CPT-II expressions in both heart and skeletal muscle. Histopathological evaluations demonstrated increased cytoplasmic lipid droplets in high-calorie fed sedentary rats, and exercise had reduced lipid droplets in skeletal muscle. Also, both mitochondria and nuclei distribution were impaired in high-calorie groups. In conclusion, aerobic exercise without food restriction was not enough to make significant changes in fat transportation mechanism into skeletal and heart muscle.