The Effect of Acute Caffeine Application on Anxiety-Like Behaviors of BALB/c and C57BL/6 Mice

Akıllıoğlu K., Dönmez Kutlu M., Boğa Pekmezekmek A., Doğan A.

ACTA PHYSIOLOGICA, vol.225, no.716, pp.36, 2018 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 225 Issue: 716
  • Publication Date: 2018
  • Doi Number: 10.1111/apha.13236
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.36
  • Çukurova University Affiliated: Yes


Aim: Effect of caffeine on anxiety-like behaviors was assessed in elevated plus maze (EPM) test on two different strain. Our hypothesis was identified as “effect of caffeine on anxiety-like behaviors in hereditary different strain is different”.

Methods: In our study, adult Balb/c and C57BL/6 mice (N=33) were used at 8-10weeks of age. Mice were administered with caffeine at dose of 50 mg/kg (0.1mL/kg body weight) and the same volume of saline intraperitoneally to control-group. Mice were subjected to EPM test 30 minutes after injection. Behaviors of mice were recorded by video-camera for 5 minutes and scored manually. A two-way analysis of variance (ANOVA) was used for statistical-analysis of data. The data that did not conform to assumptions of normality and homogeneity of variances were tested using the Kruskal–Wallis followed by post-hoc comparisons using the Mann–Whitney U. Ethical approval was obtained from ethics committee of Cukurova University.

Results: In Balb/cmice, 50mg/kg dose of caffeine caused increase in the time spent in open-arm and decrease in the time spent in closed-arm (p<0.05). In C57BL/6 mice, caffeine did not change behavioral measures in EPM. In EPM, in C57BL/6control-mice were decrease in the time of enter latency to open-arm of EPM compared to Balb/c control-mice (p<0.05). Number of total arm entries were increase in C57BL/6control-mice compared to Balb/c control-mice (p<0.05).

Conclusions: In Balb/cmice with higher anxiety-like behaviors, caffeine reduced anxiety-like behavior at dose of 50 mg/kg. Caffeine did not alter anxiety-like behaviors in C57BL/6 mice with low anxiety-like behavior.